Supplementary Figure 6: Overlap of p300 with STAT1 and STAT3 ChIP peaks. | Nature Immunology

Supplementary Figure 6: Overlap of p300 with STAT1 and STAT3 ChIP peaks.

From: Activation of naïve CD4+ T cells re-tunes STAT1 signaling to deliver unique cytokine responses in memory CD4+ T cells

Supplementary Figure 6

(a) Canonical pathway analysis of IL-6 regulated signatures in CD4+ TN and TEM cells. Statistical analysis shows the profile of canonical pathways that are common and distinct to both population after treatment with 20 ng/ul IL-6 for 30 min. The statistical significance assigned to each pathway is depicted by the size of the dot. A full list of the pathways identified is presented in Supplemental Table 3, and an interactive figure of additional information (http://jones-cytokinelab.co.uk/NI2019/sfigure6a.shtml). (b) ChIP-seq tracks shows p300 peaks for TH1(orange), TH2 (green) and TH17 (purple) CD4+ T cells overlapping with STAT1 and STAT3 peaks in sorted CD4+ TN (blue) and/or TEM (red) cells for the 8 distinct patters of STATs binding. Chromatin marks for Osm (chr11:4,219,039-4,241,349), Stat5b (chr11:100,796,667-100,852,538), Il4ra (chril217:125,551,189-125,572,632), Il21 (chr3:37,220,308-37,234,185), Plgrkt (chr19:29,352,142-29,370,256), Junb (chr:84,975,439-84,980,207), Socs1 (chr16:10,770,927-10,785,406) and Treml2 (chr17:48,289,565-48,303,569) are shown. (c) Prediction of consensus binding motifs for STAT1 or STAT3 transcription factors. Sequences were identified using MEME and STAMP/Jaspar software. Statistical significance of the motif prediction is shown as an E-value (MEME tool). Due to the small number of genes in some of the patterns, not significant motifs were found.

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