Supplementary Figure 7: Gating strategy for flow cytometry. | Nature Immunology

Supplementary Figure 7: Gating strategy for flow cytometry.

From: Activation of naïve CD4+ T cells re-tunes STAT1 signaling to deliver unique cytokine responses in memory CD4+ T cells

Supplementary Figure 7

(a) FSC vs SSC gate was used to selected live lymphocyte population. Doublet discrimination was applied when we use FSC-H vs FSC-A. After selecting CD4 positive CD25 negative population, we used CD62L and CD44 difference to select CD4+ T cells subsets. (b) After sorting a sample of each population were run to check population purity.

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