Supplementary Figure 6: Microglia-specific deletion of Card9 in Card9fl/flCx3cr1CreER+/– mice.
From: CARD9+ microglia promote antifungal immunity via IL-1β- and CXCL1-mediated neutrophil recruitment
Microglia and Ly6Chi monocytes were FACS-sorted from 24 h infected brains from Card9fl/flCx3cr1CreER–/– and Card9fl/flCx3cr1CreER+/– littermates that had been tamoxifen-pulsed 4 weeks earlier. Cells were pooled from 4-5 brains per group, and Card9 expression quantified by qRT-PCR relative to Gapdh. Data is pooled from 2 independent sorting experiments. Bar charts show the mean of the data, points represent the result from each independent sorting experiment (n = 2 independent experiments).
