Supplementary Figure 6: Flow cytometry gating schema for experimental validations.

To identify and subset the immune and stromal populations that emerged from the scRNA-seq analyses, we sorted synovial cell subsets and disaggregated synovial tissues based on markers revealed by the scRNA-seq analysis. a, Flow gating strategy for synovial fibroblasts. b, Flow gating strategy for synovial B cells. c, Flow gating strategy for synovial monocytes. See Methods for more details.