Supplementary Figure 10: Granzyme expression and cytokine production by synovial tissue CD8 T cells. | Nature Immunology

Supplementary Figure 10: Granzyme expression and cytokine production by synovial tissue CD8 T cells.

From: Defining inflammatory cell states in rheumatoid arthritis joint synovial tissues by integrating single-cell transcriptomics and mass cytometry

Supplementary Figure 10

a, RA synovial tissue samples were disaggregated, stained for surface markers and intracellular granzyme B (GZMB) and granzyme K (GZMK), and analyzed by flow cytometry. Shown are plots of GZMB versus GZMK expression by CD8 T cells from three representative tissue specimens out of eight total tissues analyzed. b, GZMK and GZMB expression patterns by HLA-DR+ CD8 T cells. c, IFNγ production by CD4 and CD8 T cells from RA synovial tissue, measured by intracellular flow cytometry after stimulation with PMA/ionomycin. Cells from the same synovial tissue sample are connected by a line. (one–tailed Student’s t test P = 0.028, t value = 2.1, df = 10.94). d, TNF production by CD4 and CD8 T cells from RA synovial tissue.

Back to article page