Supplementary Fig. 5: CD8 T cell egress from lymph nodes is dispensable for the induction of IAC, but antigen-specific activation is required.

a–d) Virus-specific CD3⁺CD8⁺ T cells of LCMV-infected and uninfected mice after daily gavage administration of either FTY720 or water. Cell were isolated from blood at day 6 (n = 5) (p-values: **** < 0.0001, **0.0083 two-way ANOVA, Bonferroni correction) (a) and day 8 post infection (n = 5) (p-values: ****<0.0001, **0.0013, **0.0012 two-way ANOVA, Bonferroni correction) (b). At day 8 post-infection, cells were also isolated from inguinal LN (n = 5) (p-values: **** < 0.0001, **0.0036, *0.0142 two way ANOVA, Bonferroni correction) (c) and spleen (n = 5) (p-values: ****<0.0001, ***0.0002 two way ANOVA, Bonferroni correction) (d). e, f) Flow cytometry analysis showing the percent of CD8⁺ T cells carrying CD45.1⁺ vs CD45.2⁺ congenic markers after bone marrow reconstitution in indicated chimeras (n = 6) (p-values: ****<0.0001 unpaired two-tailed Student’s t-test). g,h) Percentage and total number of GP33⁺CD8⁺ T cells in chimeric mice at 12 days after LCMV infection (n = 6) (p-values: **0.0038, *0.0282 unpaired two-tailed Student’s t-test). e-h) Data are pooled from two independent experiments. All data shows mean ± s.e.m.