Supplementary Fig. 1: Characterization of the pathophysiologic changes induced by IAC.
From: CD8+ T cells induce cachexia during chronic viral infection
a) Activity, oxygen consumption (VO2) and respiratory exchange ratio (RER) of LCMV-infected mice compared to uninfected controls (n = 10). Data are representative of a single experiment. b) Circulating viral load of LCMV-infected mice, measured using focus-forming assay (n = 4). Data are representative of two independent experiments. c,d) LCMV nuclear protein as measured by qPCR from tissue homogenate of inguinal, gonadal, and interscapular-brown adipose tissue up to 8 days post-infection, and 17 days post-infection (column graph) (n = 5), representative of three independent experiments (c), as well as quadriceps, gastrocnemius and soleus muscles (n = 5) representative of two independent experiments. e-g) RNA expression of myoblast differentiation 1 (MoyD1), and the proteasomal degradation markers Atrogin1 (Fbxo32) and Murf1 (Trim63) in the indicated muscle compartment (n = 5) (p-values: **0.0025, **0.0029, *0.0252, **0.0059 (e), **0.0087, **0.0071 (f), ***0.0003, *0.0295 (g) one-way ANOVA, Bonferroni correction). h) Body weight kinetics of mice infected with a titrated dose of LCMV-Cl13, ranging from 2 × 106 FFU to 2 × 102 FFU (n = 3), (p-values: **** < 0.0001, **0.0021 two-way ANOVA). i) Body weight kinetics of mice infected with high dose LCMV-Cl13 compared to LCMV-ARM (n = 4), (p-values: ****< 0.0001 two-way ANOVA). h-i) data are representative of three independent experiments. j) Body weight kinetics Of LCMV-infected mice after gavage supplementation of indicated diet between 4 and 7 days after infection (n = 4) (p-values: **0.0029, ****< 0.0001, two-way ANOVA) Data are representative of a single experiment. All data shows mean ± s.e.m.
