Supplementary Figure 1: Analysis of cells obtained from cryopreserved viable kidney tissue.
From: The immune cell landscape in kidneys of patients with lupus nephritis
a, Leukocyte yields from kidney tissue samples processed fresh or after freeze/thaw. b, Leukocyte yields from kidney tissue samples cryopreserved either post-dissociation (frozen cells) or pre-dissociation (frozen tissue). n = 9 segments pooled from 4 different donors. * p < 0.01 by two-tailed Mann-Whitney U-test. c, Cell yields and leukocyte frequencies from kidney samples either cryopreserved or shipped overnight on wet ice. Wilcoxon matched-pairs signed rank test. d, Example of flow cytometry analysis of cells from a cryopreserved lupus nephritis kidney biopsy. e, RNA-seq quality metrics of bulk leukocytes from kidney cells analyzed before (fresh cells) and after freeze/thaw (frozen cells), or obtained from kidney tissue shipped overnight or shipped after tissue cryopreservation (frozen tissue). f-h, The distribution of the number of genes per cell on each processed 384-well plate, in kidney leukocytes (f), kidney epithelial cells (g) and urine leukocytes (h).
