Supplementary Figure 2: LINK-A-dependent breast tumor represents human basal-like breast cancer.
From: Oncogenic lncRNA downregulates cancer cell antigen presentation and intrinsic tumor suppression
(a) Immunohistochemistry detection using indicated antibodies of Tg(LINK-A) mammary gland, MMTV-Tg(LINK-A) tumor or human breast cancer tissues as positive controls. Scale bar: 50 µm. Three independent experiments were performed and yielded similar results. (b) Immunoblotting detection using indicated antibodies of Tg(LINK-A) mammary gland or MMTV-Tg(LINK-A) tumors. Three independent experiments were performed and yielded similar results. (c) Missense mutation burden per Mb of MMTV-Tg(LINK-A) tumors (n = 9) and human TNBC (n = 135). (d) The presence of non-silence somatic mutations of Trp53 and Pi3kca gene, with individual tumor-bearing MMTV-Tg(LINK-A) mice are shown, with indicated mutation (red box) or without indicated mutation (white box). (e) Hierarchical clustering of mouse tumor samples (M) and human breast cancer patient samples (H) based on normalized gene expression of PAM50 gene signature. (f) Enrichment of GO biological process (BP) terms for up-regulated genes (red) and down-regulated genes in tumor versus normal samples (n = 3, 3 animals). (g-i) Log2 of fold changes of indicated metabolites in MMTV-Tg(LINK-A) breast tumor compared to that of Tg(LINK-A) mammary gland (n = 3 animals respectively).
