Supplementary Figure 7: LINK-A represses antigen presentation.
From: Oncogenic lncRNA downregulates cancer cell antigen presentation and intrinsic tumor suppression
(a) Measurement of LINK-A copy number per cell of B16F10 cells Tet-off or Tet-on LINK-A expressing followed by vehicle or IFN-γ stimulation (n.s., p > 0.05, ***p < 0.001). Results are mean ± s.d. of n = 3 independent experiments. P values were determined by one-way ANOVA. (b) RT-QPCR detection of Trim71 mRNA in B16F10 cells expressing blank vector or Trim71 expression constructs followed by vehicle or IFN-γ stimulation (n.s., p > 0.05, ***p < 0.001). Results are mean ± s.d. of n = 3 independent experiments. P values were determined by one-way ANOVA. (c) Flow cytometry detection of H-2Kb of B16F10 cells expressing blank vectors or Trim71 followed by vehicle or IFN-γ stimulation (**p < 0.001, ***p < 0.001). Results are mean ± s.d. of n = 3 independent experiments. P values were determined by one-way ANOVA. (d) Flow cytometry detection of H-2Kb of B16F10 cells expressing blank vectors or LINK-A followed by vehicle or IFN-γ stimulation and knockdown of Trim71 using LNAs in LINK-A expressing cells (*p < 0.05, ***p < 0.001). Results are mean ± s.d. of n = 3 independent experiments. P values were determined by one-way ANOVA. (e) RT-QPCR detection of LINK-A transcript in MCF7 cells harboring LINK-A expression constructs (***p < 0.001). Results are mean ± s.d. of n = 3 independent experiments. P values were determined by unpaired two-tailed Student’s t test. (f,g) Flow cytometry detection of B2M (f) or HLA-ABC (g) of MCF7 cells harboring LINK-A expression constructs (***p < 0.001). Results are mean ± s.d. of n = 3 independent experiments. P values were determined by unpaired two-tailed Student’s t test. (h) RT-QPCR detection of LINK-A transcript in MDA-MB-231 cells harboring indicated LNAs (***p < 0.001). Results are mean ± s.d. of n = 3 independent experiments. P values were determined by unpaired two-tailed Student’s t test. (i,j) Flow cytometry detection of B2M (i) or HLA-ABC (j) of MDA-MB-231 cells harboring indicated LNAs (***p < 0.001). Results are mean ± s.d. of n = 3 independent experiments. P values were determined by unpaired two-tailed Student’s t test.
