Supplementary Figure 4: Ms4a4a expression in murine leukocyte subsets and polarized BMDMs.

a) Ms4a4a mRNA levels in murine leukocyte subsets sorted form the spleen of wild-type animals (NK cells: CD45⁺/CD3^-/NK1.1⁺; CD4⁺ T cells: CD45⁺/CD3⁺/CD4⁺; CD8⁺ T cells: CD45⁺/CD3⁺/CD8⁺; B cells: CD45⁺/B220⁺; PMN: CD45⁺/CD11b^hi/Gr1^hi; mDC: CD45⁺/CD11b⁺/CD11c⁺; pDC: CD45⁺/CD11b^-/CD11c⁺; macrophages: CD45⁺/CD11b⁺/F4/80^hi). Results are shown as mean ± SEM of Ms4a4a relative expression normalized to Gapdh (n = 3 mice). Statistical analysis by one-way ANOVA. b) MS4A4A mRNA levels in wild-type BMDMs exposed or not (M0) to 20 ng/ml IFN-γ plus 100 ng/ml LPS, 20 ng/ml IL-4, 10⁻⁶ M Dex, or 20 ng/ml IL-4 plus 10⁻⁶ M Dex for 18 h. Results are shown as fold change over resting macrophages (M0; n = 5). Statistical analysis by one-way ANOVA. c, Ms4a4a mRNA levels in BMDMs from wild-type (closed symbols) or Ms4a4a^-/-(open symbols) mice treated (Dex) or not (NT) with 10⁻⁶ M Dex for 24 h. Results are shown as mean ± SEM of Ms4a4a relative expression normalized to Gapdh (n = 3 mice). Statistical analysis by two-tailed unpaired (Mann-Whitney) Student’s t test and one-way ANOVA. d) Percentage of NK cells (CD45⁺/CD3^-/NK1.1⁺), T cells (CD45⁺/CD3⁺), B cells (CD45⁺/B220⁺), PMN (CD45⁺/CD11b^hi/Gr1^hi), mDC (CD45⁺/CD11b⁺/CD11c⁺), pDC (CD45⁺/CD11b^-/CD11c⁺), and macrophages (CD45⁺/CD11b⁺/F4/80^hi) in the spleen of wild-type (closed symbols) or Ms4a4a^-/- (open symbols) mice. Results are shown as mean ± SEM (n = 4). No statistical difference by two-tailed unpaired Student’s t test.