Supplementary Figure 6: Ms4a4a impact on Dectin-1 signalling in BMDMs.

a–c) Syk phosphorylation in BMDMs from Ms4a4a^-/- (open symbol) and wild-type (closed symbol) mice primed for 18 h with 10 ng/ml GM-CSF and stimulated or not with 100 μg/ml depleted zymosan (a), 100 μg/ml curdlan (b), or 100 ng/ml PMA (d). Results are shown as mean ± SEM of relative MFI (fold on untreated). Three independent experiments were performed. a) time 5: 4 wild-type and 4 Ms4a4a^-/- mice; time 15: 5 wild-type and 8 Ms4a4a^-/- mice. b) time 5: 6 wild-type and 5 Ms4a4a^-/- mice; time 15: 5 wild-type and 6 Ms4a4a^-/- mice. c) time 5: 4 wild-type and 5 Ms4a4a^-/- mice; time 15: 4 wild-type and 4 Ms4a4a^-/- mice. Statistical analysis by two-tailed unpaired (Mann-Whitney) Student’s t test. d, e) Phosphorylation of ERK (d) and p38 (e) in BMDMs from Ms4a4a^-/- (open symbols) and wild-type (closed symbols) mice primed for 18 h with 10 ng/ml GM-CSF and stimulated or not (NT) with 100 μg/ml depleted zymosan, 100 μg/ml curdlan, 100 ng/ml LPS, and 100 ng/ml PMA. Results are shown as mean ± SEM of relative MFI (fold on untreated). Two independent experiments were performed. One dot represents one mouse (2–4 for e, 2–6 for f). f–k) Secretion of IL-6 (f–h) and TNF (i–k) by BMDMs from Ms4a4a^-/- (open symbol) and wild-type (closed symbol) mice primed for 18 h with 10 ng/ml GM-CSF and stimulated for 24 h (f, g and i, j) or 6 h (h–k) with 100 μg/ml depleted zymosan (f–i), 100 μg/ml curdlan (g–j) or 100 ng/ml Pam3Cys (h–k). Cytokine levels in untreated cells were below detection limit. Results are shown as mean ± SEM. Two independent experiments for f and i (9 wild-type and 11 Ms4a4a^-/- mice for f; 8 wild-type and 12 Ms4a4a^-/- mice for i); five independent experiments for g and j (14 wild-type and 16 Ms4a4a^-/- mice for g; 13 wild-type and 16 Ms4a4a^-/- mice for j), three independent experiments for h and k (7 wild-type and 10 Ms4a4a^-/- mice). Statistical analysis by two-tailed unpaired (Mann-Whitney) Student’s t test.