Supplementary Figure 3: Workflow and Quality Control for sorted single BM progenitors.

a Experimental approach for analysis of gene expression in single CLPs, GMPs, LMPPs, and MDPs using the Fluidigm C1 autoprep system. Violin plots for b number of genes detected in each single cell, c nUMI and d and percentage of mitochondrial genes in the sorted single cell populations. e Bar plot of number of expressed genes in individual sorted single cells. Data are representative for one experiment with 63 single LMPPs, 51 single GMPs, 48 single CLPs, 59 single MDPs. f Flow cytometry analysis of surface molecule expression of CD81 and Ly6D on Ly6C^-SiglecH^- pre-DCs, Ly6C⁺SiglecH^- pre-DCs, SiglecH⁺Ly6C⁺ pre-DCs, and SiglecH⁺Ly6C^- pre-DCs. Data are representative of three independent experiments with three mice. g Venn diagram comparison of pre-pDC primed DEGs shared between CLPs and SiglecH⁺Ly6C^- pre-DCs for g least 50 % or h 10% of cells per population. g,h Data are representative for one experiment with 48 single CLPs, and 70 single SiglecH⁺Ly6C^- pre-DCs.