Supplementary Figure 4: PD-1+CD38hi CD8+ T cells induced as a result of anti-PD-1 pre-treatment are dysfunctional. | Nature Immunology

Supplementary Figure 4: PD-1+CD38hi CD8+ T cells induced as a result of anti-PD-1 pre-treatment are dysfunctional.

From: PD-1 blockade in subprimed CD8 cells induces dysfunctional PD-1+CD38hi cells and anti-PD-1 resistance

Supplementary Figure 4

a-b. Frequency of CD40L+ (a) and IFN-γ+ (b) T cells in PD-1+CD38hi CD8+ T cell population. c-d. Frequencies of Annexin V+ PD-1+CD38hi cells in total (c) and antigen-specific (d) CD8+ T cells at D13 post-B16 tumor implantation. Data are the average of two independent experiments. Each dot corresponds to one mouse with the indicated number of mice per group given in parentheses. The aerror bars indicate the s.e.m. For comparison purposes, an unpaired, one-tailed Student’s t-test was used. NSnon-significant (a) *(lower) p=0.0463, *(upper) p=0.0496, **p=0.0086, ***p=0.0009; (b) *(lower) p=0.018, *(upper) p=0.0441, **p=0.01, ****p≤0.0001; (c) vs. UT: *(lower) p=0.0327 and *(upper) p=0.0275, vs. Vax: *(lower) p=0.0143 and *(upper) p=0.0273, ****p≤0.0001; (d) *(lower) p=0.026, *(upper) p=0.0382, **p=0.01, ****p≤0.0001. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.

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