Supplementary Figure 2: LPS exposure modulates mitochondrial respiration and glucose utilization as a function of time to support inflammatory cytokine production.
From: Glycerol phosphate shuttle enzyme GPD2 regulates macrophage inflammatory responses
(a) Seahorse extracellular flux analysis of maximal mitochondrial oxygen consumption rate (OCR) in BMDMs left unstimulated or stimulated with LPS for the times indicated (n=4). (b) 3H-deoxy-D-glucose uptake (cpm = counts per min) in BMDMs left unstimulated or stimulated with LPS for the times indicated (n=3). (c) ChIP-qPCR analysis of histone acetylation in Il6 and Il1b promoter regions in BMDMs stimulated with LPS for 0-1h in media +/- glucose, following 24h incubation in media +/- glucose (n=3). (d) qPCR analysis of Il6 and Il1b gene expression in BMDMs stimulated with LPS for 0-3h in media +/- glucose, following 24h incubation in media +/- glucose. Data are one experiment representative of ten (a) or three (b-d) independent experiments. Mean values (a-d) +/- s.e.m. (a,b) shown. *p ≤0.05, **p ≤0.01, ***p ≤0.001 (two-tailed Student’s t-test).
