Supplementary Figure 9: Myd88 deficiency restores the steady-state transcriptional program in Spop KO LSKs. | Nature Immunology

Supplementary Figure 9: Myd88 deficiency restores the steady-state transcriptional program in Spop KO LSKs.

From: The E3 ubiquitin ligase SPOP controls resolution of systemic inflammation by triggering MYD88 degradation

Supplementary Figure 9

a, Representative flow cytometry analysis plots of the proportion of HSPC in the lineage- bone marrow from hematopoietic chimeric mice following Poly(I:C) injection including: control (Spop+/+MxCre MyD88+/+), Spop KO (SpopΔ/ΔMxCre MyD88+/+), Myd88 KO (Spop+/+MxCre MyD88-/-) and dKO (SpopΔ/ΔMxCre MyD88-/-. Data are representative from 2 independent experiments. b, Spop mRNA expression levels of LSKs on d10 post Poly (I:C) injection (n=2). c, Immunoblot analysis of HSPC cKit+ bone marrow cells of wild-type (Spop+/+MxCre MyD88+/+), Spop KO (SpopΔ/ΔMxCre MyD88+/+) and dKO (Spop+/+MxCre MyD88-/-) mice. Data are representative from 3 independent experiments. d, Heatmap showing the expression levels of the 50 most significant markers per cluster, displaying 100 randomly-selected cells. e, Percentage of Cycling cells per genotype. f, Percentage of myeloid Cd11b+Ly6G+ cells in peripheral blood of SpopΔ/ΔMxCre mice on day 20 following pIpC challenge and antibody treatment. Bar plots represent mean+-s.d, n=4 mice per condition. g, Representative Flow Cytometry Analysis Plots of the proportion of Myeloid (Cd11b+, Ly6G+) cells in the peripheral blood of the pIpC-stimulated mice.

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