Supplementary Figure 1: Spop is highly expressed in LT-HSC.

a, Top 15 highest expressed ubiquitin ligase genes in LT-HSC. Heatmap represents the normalized counts relative to 10M reads per gene in the indicated populations. The data was collected from Lara-Astiaso., 2016 ²⁹ b, Spop expression profile on different hematopoietic cells from the same dataset. c, Immunoblot showing Spop protein levels in the indicated sorted cells. Data are representative from two independent experiments d, Schematic diagram of Spop conditional allele. e, Immunoblot analysis of Spop protein levels of total bone marrow cells from wild-type (Spop^+/+MxCre) and KO (Spop^Δ/ΔMxCre) mice following a single Poly(I:C) injection. Data are representative of three independent experiments. f, Percentage of mouse weight-change on d15 after Poly (I:C) injection (Spop^+/+MxCre: n=9; Spop^Δ/ΔMxCre: n=10). Data represent minimum, first quartile, mean, third quartile and maximum. Statistical analysis: unpaired t-test, two-tailed). g, Kaplan-Meier analysis of survival of Spop^+/+MxCre and Spop^Δ/ΔMxCre hematopoietic chimeras after donor hematopoietic reconstitution and one injection of Poly(I:C) (n=5 wild-type, n=6 Spop KO. Statistical analysis: Mantel-Cox test). h, Hematoxylin & Eosin stained sections of spleen, lung and liver of wild-type (Spop^+/+MxCre) and KO (Spop^Δ/ΔMxCre) hematopoietic chimeric mice on d21 post Poly (I:C) injection. Scale bars indicate 100 um (Spleen and liver), 1000 um (lung) and 10 um magnifications. Data are representative from 3 independent experiments(pI:C: Poly (I:C). LT-HSC: Lin^-c-Kit⁺Sca-1⁺Flk2^-CD34^-. ST-HSC: Lin^-c-Kit⁺Sca-1⁺Flk2^-CD34⁺. MPP: Lin^-c-Kit⁺Sca-1⁺Flk2⁺CD34⁺. CMP: Lin^- c-Kit⁺ Sca-1⁺ FcgRII^lowCD34⁺. GMP: Lin^-c-Kit⁺Sca-1⁺FcgRII^highCD34⁺. MEP: Lin^-c-Kit⁺Sca-1⁺FcgRII^-CD34^-. CLPs: Lin^-Flk2⁺Il7R⁺).

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