Supplementary Figure 1: Binding, neutralizing and ADE activities of outbred CD1 mouse sera following immunization with ZIKV E-monomer and E-dimer proteins.

Three groups of female CD1 mice (n=6 animals per group) were immunized with E-dimer, E-monomer, or CHIKV E2 as before, using Addavax as adjuvant. (a) ELISA analysis showing the antibody titres obtained from ZIKV E-monomer (left panel), ZIKV E-dimer (middle panel) and CHIKV E2 (right panel), immunized-mouse serum on captured ZIKV and DENV 1-4 virions (for each virus, n=6 individual mouse serum per group). (b) FRNT₅₀ titres against ZIKV and DENV 1-4 were calculated for animals immunized with ZIKV E-monomer (left panel), ZIKV E-dimer (middle panel) and CHIVK E2 (right panel) (for each virus, n=6 individual mouse serum per group); in (a-b) individual measurements, as well as arithmetic mean±s.d. values are shown. (c-d) Infection enhancement curves (FFU/ml) of U937 cells infected with ZIKV PF13 (c) and DENV-2 16681 (d) viruses in presence of serially-diluted mouse immune serum (n=6 individual mouse serum per group). The data are shown as single data points and arithmetic mean.