Supplementary Figure 2: Depletion of specific antibody populations from mouse immune sera.

Serum was collected from BALB/c mice 10 weeks post prime, pooled by group and incubated with a series of antigen-displaying yeast to remove specific fractions of antibodies. The depleted sera were tested by flow cytometry for any remaining reactivity and additional rounds of depletion were performed until all the antigen-specific activity was removed. Flow cytometry profiles showing the reactivity of each sera before (red) and after (blue) depletion with each antigen displaying-yeast are shown; histogram plots were used to facilitated comparison and a negative control using mock yeast (black) was added to demonstrate complete depletion of antibody. The data shown is representative of three independent experiments performed for each depletion and sera.