Supplementary Figure 6: Ptpn2-deleted CD8+ T cells are more activated in response to MC38-OVA tumors.
From: PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity
(a) Gating strategy for analyzing transferred OT-1 CD8+ T cells from the tumor, tumor-draining lymph node, and spleen following tumor challenge. (b) Quantification of CD25 expression in OT-1 CD8+ T cells day 7 post MC38-OVA implantation in the tumor-draining lymph node for control and Ptpn2-deleted co-transferred cells as in Fig. 6a. Representative of two independent experiments, n = 7 mice. (c) Quantification of CD127 expression in OT-1 CD8+ T cells day 7 post MC38-OVA implantation in the tumor-draining lymph node for control and Ptpn2-deleted co-transferred cells as in Fig. 6a. Representative of two independent experiments, n = 7 mice. (d) Quantification of IFN-γ and TNF cytokine expression in co-transferred OT-1 CD8+ T cells in tumors on day 7 post MC38-OVA implantation for co-transferred control and Ptpn2-deleted cells as in Fig. 6a. Representative of two independent experiments, n = 6 mice. Bar graphs represent mean and error bars represent standard deviation. Statistical significance was assessed by two-sided Student’s paired t-test (b-d) (ns p>.05, * p≤.05, ** p≤.01, *** p≤.001, **** p≤.0001).
