Supplementary Figure 7: Deletion of Ptpn2 induces a systemic cytotoxic CD8+ T cell response following tumor challenge.
From: PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity
(a) Quantification of Vex expression from control or Ptpn2-deleted chimeras. Representative of two independent experiments, n = 9 mice. (b) Quantification of Ly6chi, MHC-II+, and Foxp3+ subsets in control or Ptpn2-deleted chimeras at steady state. Representative of two independent experiments, n ≥ 8 mice. (c) Quantification of inflammatory cytokines in the serum of control or Ptpn2-deleted chimeras at steady state. Nd: not detectable. Representative of two independent experiments, n = 10 mice. (d) Survival curves from Fig. 7b experiment. Representative of two independent experiments, n ≥ 8 mice. (e) Quantification of immune infiltrate in MC38 tumors 9 days post implantation in control or Ptpn2-deleted chimeras. Representative of two independent experiments, n ≥ 7 mice. (f) Quantification of CD127 expression in control or Ptpn2-deleted chimeras day 14 post MC38 tumor implantation. Representative of two independent experiments, n ≥ 7 mice. (g) Quantification of Slamf6+ and Tim-3+ subsets in control or Ptpn2-deleted chimeras day 12 post MC38 tumor implantation. Representative of two independent experiments, n ≥ 8 mice. (h-i) (h) Representative flow cytometry plots and (i) quantification of CD44 and CD62L subsets in control or Ptpn2-deleted chimeras day 14 post MC38 tumor implantation. Representative of two independent experiments, n = 5 mice. (j) Quantification of CD8+ T cells in control or Ptpn2-deleted chimeras treated with isotype or CD8-depleting antibody day 10 post MC38 tumor challenge. Representative of two independent experiments, n ≥ 4 mice. (k-l) Tumor growth curves for control or Ptpn2-deleted chimeras (k) untreated or (l) treated with a CD8-depleting antibody or isotype control rechallenged with 5 x 106 MC38-WT tumor cells, 60-days post primary MC38 tumor clearance. Representative of two independent experiments, (k) n ≥ 7 mice, (l) n ≥ 4 mice. (m) Survival curves from Fig. 7i experiment. Representative of two independent experiments, n ≥ 10 mice. Bar graphs represent mean and error bars represent standard deviation (except k-l which represent standard error). Statistical significance was assessed by two-way ANOVA (b-c, e, g, i, k-l), two-sided log-rank Mantel–Cox test (d, m), or by two-sided Student’s unpaired t-test (f) (ns p>.05, * p≤.05, ** p≤.01, *** p≤.001, **** p≤.0001).
