Supplementary Figure 1: Ptpn2-deleted naive CD8+ T cells do not express effector molecules at baseline or polyfunctional cytokines following infection.
From: PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity
(a) Representative flow cytometry plots of CD44 and CD62L expression on naive control or Ptpn2-deleted P14 CD8+ T cells used in co-transfer experiments. Representative of two independent experiments, n = 1 mouse. (b-d) Representative flow cytometry plots of (b) Granzyme B expression, (c) Ki-67 expression, and (d) BrdU incorporation on naive control or Ptpn2-deleted P14 CD8+ T cells used in co-transfer experiments. Representative of two independent experiments, n = 5 mice. (e) Gating strategy for analyzing co-transferred CD8+ T cells from the spleen following LCMV Clone 13 infection as in Fig. 1c. (f) Quantification of frequency of IFN-γ+ TNF+ CD8+ T cells of co-transferred control and Ptpn2-deleted P14 T cells on days 8, 15, 22, and 30 post LCMV Clone 13 infection. Representative of two independent experiments, n ≥ 4 mice. Bar graphs represent mean and error bars represent standard deviation. Statistical significance was assessed by two-sided Student’s paired t-test (f) (ns p>.05, * p≤.05, ** p≤.01, *** p≤.001, **** p≤.0001).
