Supplementary Figure 2: Deletion of Ptpn2 decreases the frequency of cells expressing progenitor-associated markers.
From: PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity
(a) Representative flow cytometry plots of Tim-3 and Slamf6 expression on naive control or Ptpn2-deleted P14 CD8+ T cells used in co-transfer experiments. Representative of two independent experiments, n = 1 mouse. (b) Representative flow cytometry plots of Tim-3 and CXCR5 expression on control or Ptpn2-deleted P14 T cells in the spleen 8 days post LCMV Clone 13 infection. Representative of two independent experiments, n = 5 mice. (c) Frequency of Tim-3+ CXCR5– and Tim-3– CXCR5+ control or Ptpn2-deleted P14 T cells in the spleen 8 days post LCMV Clone 13 infection. Representative of two independent experiments, n = 5 mice. (d) Frequency of TCF1 and CD127 expressing control or Ptpn2-deleted P14 T cells in the spleen 8 days post LCMV Clone 13 infection. Representative of two independent experiments, n = 5 mice. (e) Number of Tim-3+ CXCR5– and Tim-3– CXCR5+ control or Ptpn2-deleted P14 T cells in the spleen 8 days post LCMV Clone 13 infection. Representative of two independent experiments, n = 5 mice. (f) Quantification of Tim-3+ Slamf6– and Tim-3– Slamf6+ subsets 8 days post LCMV Clone 13 infection of non-TCR transgenic control or Ptpn2-deleted bone marrow chimeras. Subsets in (f) are pre-gated on PD-1 to identify responding cells and Vex. Representative of two independent experiments, n = 3 mice. Bar graphs represent mean and error bars represent standard deviation. Statistical significance was assessed by two-sided Student’s paired t-test (c-e) or two-way ANOVA (f) (ns p>.05, * p≤.05, ** p≤.01, *** p≤.001, **** p≤.0001).
