Supplementary Figure 13: Flow cytometry analysis of T cells from mice with human A20 OTU domain alleles.

(a) Cumulative data showing CD4⁺FOXP3⁺ regulatory T cells from spleens of T108A;I207L (I207L), I325N or C243Y mice. Each point indicates an individual mouse collected form 3 independent experiments (I207L and C243Y) or 4 independent experiments (I325N). Statistical analysis by 2-tailed Student’s t-test, data shown as mean ±s.e.m, **P < 0.01, ****P < 0.0001. (b, c) Representative flow cytometric analysis of splenocytes from mice of the indicated genotypes, showing percentages of CD4⁺ or CD8⁺ T cells, and the percentage of CD44^hi activated/memory cells within these subsets, and the percentage of CD25⁺CD44^int regulatory T cells or CD25^- CD44^hi effector/memory T cells among CD4⁺ cells. Data is representative of the same 3 independent experiments conducted in (a).