Supplementary Figure 14: Flow cytometry analysis of human T108A;I207L peripheral blood mononuclear cells.

(a) Gating strategy used to analyse human peripheral blood mononuclear cell data collected by flow cytometry. Arrows used to illustrate the step wise analysis of cell populations identified on the basis of physical properties and surface marker staining. Heading titles above each plot indicates gated cell population analysed in that plot. (b-d) Peripheral blood mononuclear cells from individuals heterozygous (green circles) or homozygous (purple circle) for the Denisovan TNFAIP3 haplotype (hap) and 6 age-matched healthy control donors (HCs; blue circles), were assessed by flow cytometry for percentages of CD4⁺CD25^hiCD127^lo regulatory T cells (b), different subsets of non-regulatory CD4⁺CD25^loCD127^hi/lo T cells (c), and CD8⁺ T cell subsets (d). (CM=central memory; EM=effector memory; EMRA = terminally differentiated effector memory cells). Bars show mean ± s.d. Two-tailed Student’s t-test was used to test for significance, only significant differences are indicated, *P < 0.05.