Supplementary Figure 6: Peripheral T cells ectopically expressing the mutant G135D LAT exhibit a lower responsive threshold while stimulated with weak ligands or self-peptides.
From: Slow phosphorylation of a tyrosine residue in LAT optimizes T cell ligand discrimination
Flow cytometric analysis of CD69 upregulation and mCherry expression in GFP+Vα2+CD8+ T cells after stimulation with different peptide-pulsed splenocytes (as indicated on top of the contour plots) as in Fig. 5. The GFP+ mCherry-negative population did not express the transduced LAT and, thus, did not respond to peptide stimulation. Data are representative of three experiments.
