Supplementary Figure 3: Altered LN and PP cellularity is not a consequence of dehydration.
From: Mechanosensing by Peyer’s patch stroma regulates lymphocyte migration and mucosal antibody responses
(a) Flow cytometric analysis of total lymphocyte counts from PP and LN of mice after the following treatments: control untreated, PEG for 3 days, PEG for 3 days then restored to normal drinking water for 12 hours (PEG > Water). (n=11 (Ctl, PEG) or 12 (PEG > Water) mice per group). (b) Flow cytometric analysis of total lymphocyte counts from PP and LN after the following treatments: control untreated, PEG for 3 days, amiloride for 3 days, PEG or amiloride for 3 days with twice daily subcutaneous injection of saline. (n = 13 (Ctl, PEG) or 8 (Aml) mice per group). Data represented as mean ± SEM. Groups were compared by one way ANOVA. * (P ≤ 0.05), ** (P ≤ 0.01), *** (P ≤ 0.005). No statistical significance is indicated with ‘ns’.
