Supplementary Figure 5: Specific expression of Ccl19cre transgene in the FRC population.
From: Mechanosensing by Peyer’s patch stroma regulates lymphocyte migration and mucosal antibody responses
(a,b) Confocal imaging analysis of the distribution of eYFP-expressing cells in the intestinal PP of Ccl19cre+ x ROSA26-eYFP mice. Representative of three experiments (a) FDCs are identified by positive staining with anti-CR1/CR2 (blue); MRCs are identified by positive staining with anti-RANKL (Red); FRCs are identified by the positive staining with anti-PDPN (grey) and absence of staining with anti-CR1/CR2 and anti-RANKL. Left image scale bar = 100um. Right images scale bar = 20um. (b) Representative confocal image of an HEV in cross-section. HEV endothelial cells are identified by positive staining with anti-MAdCAM (red). Scale bar = 10um.
