Supplementary Figure 1: Stromal cell numbers in PPs following treatments with PEG or amiloride. | Nature Immunology

Supplementary Figure 1: Stromal cell numbers in PPs following treatments with PEG or amiloride.

From: Mechanosensing by Peyer’s patch stroma regulates lymphocyte migration and mucosal antibody responses

Supplementary Figure 1

(a,b) Flow cytometric analysis PP stromal cell populations (CD45−TER119−) following treatment with PEG or amiloride. Subpopulations are defined as FRC (PDPN+CD31−), LEC (PDPN+CD31+), BEC (PDPN−CD31+, and HEV (CD31+MAdCAM+). Mice were treated for a period of 3 days (a; n = 6 mice per group) or one week (b; n = 15 (Ctl, PEG) or 5 (Aml) mice per group). Representative FACS plots illustrate relative composition of FRCs (gp38+CD31−), LECs (gp38+CD31+), and BECs (gp38−CD31+). Data are graphed as relative cell counts. For all graphs, data represented as mean ± SEM. Groups were compared by one way ANOVA. *P < 0.05, **P < 0.01, ****P < 0.0001. No statistical significance is indicated with ‘ns’.

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