Extended Data Fig. 2: Ectopic expression of NIK improves CD8 T cell function in adoptive T cell therapy.
From: NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity
a,b, Flow cytometric analysis of the frequency of total donor CD8 T cells (a) and IFNγ-producing donor CD8 T cells (b) in the tumor of B16F10 melanoma-bearing B6.SJL recipient mice adoptively transferred with wildtype or NIKiTg Pmel1 CD8 T cells as described in Fig. 2o (n = 4 per genotype). c, In vitro cytotoxicity assay of wildtype (WT) and NIKiTg Pmel1 CD8 effector T cells towards B16F10 tumor cells at the indicated effector to tumor (E:T) ratios. d-f, Schematic of experimental design (d) and flow cytometric analysis of apoptosis based on caspase 3 cleavage (e) or proliferation based on CFSE dilution (f) of tumor-infiltrating wildtype (WT) and NIKiTg OT-I CD8 T cells in B16-OVA-tumor bearing B6.SJL mice adoptively transferred with in vitro activated and CFSE-labeled WT or NIKiTg CD8 T cells for 7 days (e, n = 3 per genotype). Data are representative of two independent experiments. Summary data are shown as mean ± s.e.m. with P values determined by two-tailed Student’s t test.
