Extended Data Fig. 6: Noncanonical NF-κB activation is not required for HK2 stabilization or T cell metabolism.
From: NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity
a, Immunoblot analysis of HK2 expression and p100 processing (as a measure of noncanonical NF-kB activation) in WT or Nfkb2Lym1/+ (Lym1/+) OT-I CD8 T cells stimulated with anti-CD3 plus anti-CD28 for the indicated time periods. b,c, Seahorse analysis of basal ECAR (after glucose injection) and maximum ECAR (after oligomycin injection) (b) and Seahorse analysis of baseline OCR (no treatment) and maximum OCR (FCCP injection) (c) of WT or Nfkb2Lym1/+ (Lym1/+) OT-I CD8 T cells, either naïve or activated with anti-CD3 and anti-CD28 for 24 h, under baseline and stressed conditions. d, Coimmunoprecipitation analysis of NIK-HK2 interaction using whole-cell lysates of 293 cells transfected with (+) or without (–) the indicated expression vectors. Data are representative of three independent experiments. Summary data are shown as mean ± s.e.m. based on multiple wells (each circle represents a well) with P values determined by two-tailed Student’s t test.
