Extended Data Fig. 5: Mitochondrial activity identifies Vγ4+ progenitors with distinct effector fates at very early stages. | Nature Immunology

Extended Data Fig. 5: Mitochondrial activity identifies Vγ4+ progenitors with distinct effector fates at very early stages.

From: Distinct metabolic programs established in the thymus control effector functions of γδ T cell subsets in tumor microenvironments

Extended Data Fig. 5

a, Flow cytometry plots pre-sort, and after sorted TMRElo and TMREhi Vγ4+γδ24+ cells were cultured for 5-days on OP9DL1 cells. Percentage of thymic γδ17 and γδIFN cells generated are displayed in the graph on right. Data are representative of 3 independent experiments (cells sorted from n = 4 independent mice pooled per group per experiment). b, Flow cytometry plots for pre- and post-sort TMREhi and TMRElo Vγ4+γδTN cells that were cultured on OP9-DL1 cells for a further 5-days (plots on right). Histogram shows the percentage of each γδ T cell subset generated from cultured TMRElo and TMREhi Vγ4+γδTN cells. Error bars show mean + SD. Data are representative of 2 independent experiments (at least 4 lobes pooled per group per experiment). Error bars show mean±SD, *p < 0.05, ***p < 0.01, using two-tailed unpaired Student’s t-test.

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