Extended Data Fig. 1: SCENITHTM methodology for analysis of cell metabolism.
a, Experimental design: E0771 breast or MC38 colon cancer cell lines were injected in WT mice; 6 and 15 days later, tumors were extracted for metabolic analysis of gd T cells using SCENITHTM. b, SCENITHTM assesses the impact of metabolic inhibitors on protein synthesis. Mean fluorescence intensity (MFI) of puromycin is analysed in each condition (Co: control-no inhibition; DG: 2-deoxyglucose inhibiting glycolysis; O: oligomycin inhibiting OXPHOS; and DGO: DG+O inhibitors). Glucose dependence, fatty acid and amino acid oxidation capacity, mitochondrial dependence and glycolytic capacity are calculated as detailed in the Methods and reference #23. Error bars show mean+SEM. Data are representative of 3 independent experiments (n=3 mice in triplicates per group and per experiment).
