Extended Data Fig. 3: WT and ZF7 T cell signaling and T cell profiling.
From: Non-catalytic ubiquitin binding by A20 prevents psoriatic arthritis–like disease and inflammation
a, Flow cytometry analysis of TCRβ+ and TCRδ+ T cells in lymph nodes (axillary, brachial and inguinal combined, left) and total quantitation of TCRδ+ cells in lymph nodes (right). b, (left) Representative flow cytometry analysis of naive CD4+ T cells after separation from spleen and lymph nodes (EasySep Mouse Naive CD4+ T cell Isolation Kit). (right) Immunoblot analysis of TCR signaling from purified naive CD4+ T cells shown at left. Purified naive CD4+ T cells were stimulated with anti-CD3 mAb (145-2C11; Tonbo) followed by crosslinking with goat anti-hamster IgG (Jackson Immuno) for the indicated times at 37 °C with gentle shaking. Data are representative of three independent experiments.
