Extended Data Fig. 7: Extended data on the murine delayed-type hypersensitivity model.

a, Spleen weight following mBSA-driven DTH response in young WT (Y WT, n = 4 mice), old WT (O WT, n = 8 mice), old Sesn1^−/− (O Sesn1^−/−, n = 5 mice), and old Sesn2^−/− (O Sesn2^−/−, n = 4 mice). Bars represent means and s.d.. b, Representative gating strategy to identify NK1.1⁺ NK cells (violet), TCRβ⁺CD1d tetramer reactive iNKT cells (purple), TCRβ⁺CD3⁺ CD4⁺ (blue) and CD8⁺ (red) T cells in mice. Similar results were obtained in all mice (n = 3 per group). c, Quantification of these cell types in the spleen (means and s.e.m., n = 3 mice per group). d, Dot plots showing relative frequencies of CD44⁻CD62L⁺ naive (gray), CD44⁺CD62L⁺ central (blue), and CD44⁺CD62L⁻ effector (red) CD8⁺ T cells. e, Quantification of these cell types as a proportion of total splenic CD8⁺ T cells (means and s.e.m., n = 3 per group). f, Enumeration of NKG2D, NKG2A/C/E, KLRG1, Ly49, and NKp46 expression on CD8⁺ T cells from Y WT, O WT, O Sesn1^−/−, and O Sesn2^−/− mice (means and s.e.m., n = 3 per group). One-way ANOVA with Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.