Extended Data Fig. 1: The frequency of CD8-MP cells is not diminished in germ-free mice.
From: Eomes identifies thymic precursors of self-specific memory-phenotype CD8+ T cells
a, Representative flow-cytometric analysis of CD44 vs. CD122 expression by CD8β+ T cells from the spleens of 16-week-old B6 specific pathogen free (SPF) and germ-free (GF) mice. The percentage of cells falling in the indicated gates is denoted. Data are representative of two independent experiments. b, Summary plot of the frequency of CD44hiCD122+ expressing TCRβ+ CD8β+ T cells from the indicated lymphoid sites in 6 to 16-week-old SPF or GF mice. iabLN: inguinal, axial, brachial lymph nodes; cLN: cervical lymph nodes; mLN: mesenteric lymph nodes; pLN: periaortic lymph nodes. Each symbol represents an individual mouse. Mean ± SEM is indicated. n = 6, Spleen, iabLN, cLN, mLN; n = 3, pLN. At each lymphoid site, the frequency of CD8-MP cells was compared between the SPF and GF mice using one-way ANOVA with Bonferroni post-test analysis, comparing all pairs of columns (ANOVA p < 0.0001, F = 17.43, df = 53). Adjusted p-values from the Bonferroni post-test are depicted: Spleen, *p = 0.0133; iabLN, n.s. p = 0.3669; cLN, n.s. p > 0.9999; mLN, n.s. p > 0.9999; pLN, n.s. p > 0.9999. Data are pooled from two independent experiments. (n.s., not significant).
