Extended Data Fig. 2: Bcl-6 exhibits direct negative autoregulatory feedback.

Related to Fig. 2. a, Sequence homology at BPS1 locus between various species. The numbers indicate region of mouse chromosome 19 (mm10). b, Schematic diagram of self-inactivating (SIN) vectors. pQdT SIN vector were generated from original pQCXIP SIN vector by removing IRES-Puro^R cassette and additional deletion of CCAAT box and TATA box. c, Schematic of the RV⁺ SMARTA cell transfer system used for LCMV_Arm infection. SMARTA CD4 T cells transduced with WT Thy1.1-RV or ΔBPS1 Thy1.1-RV were adoptively transferred to C57BL/6 host mice, followed by infection of the host mice with LCMV_Arm, and analyzed 7 days after infection. Related to Fig. 2a-b and Extended Data Fig. 2d-e. d, T_FH and T_H1 gates used for Fig. 2b and Extended Data Fig. 2e. e, Chromatogram of DNA sequencing from WT or ΔBPS1 mouse. f, Schematic of the SMARTA cell transfer system used for LCMV_Arm infection. WT or ΔBPS1 SMARTA CD4 T cells were transferred to C57BL/6 host mice, followed by infection of the host mice with LCMV_Arm, and analyzed 7 days after infection. Related to Fig. 2c-d. g, Quantification of expression of CXCR5, SLAM, and PSGL1 in Fig. 2d, gated on CXCR5^hiSLAM^lo T_FH or CXCR5^loSLAM^hi T_H1 cells. Two independent experiments were performed; each dot represents one mouse (n = 4). Data are mean ± s.d., unpaired two-tailed Student’s t-test. h, Genome-browser tracks depict BCL-6 or NCOR ChIP–Seq peaks at BCL6 locus from OCI-LY1 cell line. Peak calls indicated below each track.