Extended Data Fig. 2: Fate-mapping of Hobit-expressing cells identifies primary TRM cells.
From: Tissue-resident memory CD8+ T cells shape local and systemic secondary T cell responses
a, Schematic representation is shown of the experimental setup using HR OT-I mice crossed onto Rosa26-flox-STOP-flox-eYFP (Rosa26eYFP) reporter mice for fate mapping of TRM cells during secondary responses. (b–e) The phenotype of adoptively transferred HR × Rosa26eYFP OT-I T cells was analyzed at >30 days after oral infection with L.m.-OVA. b, Representative flow cytometry plots show expression of YFP and tdTomato by TCM (CD69– CD62L+) and TEM (CD69– CD62L–) cells in spleen, and TRM cells (CD69+ CD62L–) in liver, SI IEL and LPL. c, The frequency of YFP+ expression was quantified within the indicated populations of memory HR × Rosa26eYFP OT-I T cells. d, The expression levels (geoMFI) of tdTomato within the indicated populations of memory HR × Rosa26eYFP OT-I T cells were quantified. Two-way ANOVA with Tukey’s multiple comparisons test, ***P < 0.0001. e, The frequencies of CD69+, CXCR6+ and CD62L+ expression within the indicated populations was quantified. One-way ANOVA with Tukey’s multiple comparisons test, *P = 0.0131, **P < 0.01, ***P < 0.0001. Combined data is shown from two independent experiments (n = 8 or 11 mice). Symbols represent individual mice; bars represent the mean. Error bars represent mean ± SEM.
