Extended Data Fig. 1: Expression of reporter protein tdTomato reflects Hobit expression.
From: Tissue-resident memory CD8+ T cells shape local and systemic secondary T cell responses
a, Exemplary gating strategy for the analysis of OT-I T cells. b, c, HR × Rosa26eYFP naïve and memory OT-I T cells were FACS-purified from spleen liver and small intestine at >30 days after oral infection with L.m.-OVA. The expression of Hobit mRNA in CD44lo CD62L+ TN cells, CD69– CD62L+ TCM cells, tdTomato– (Tom-) CD69– CD62L– TEM cells from spleen and liver, and tdTomato+ (Tom + ) CD69+ CD62L– TRM cells from liver and small intestine (SI, SI LPL and SI LPL combined) was determined via (b) qPCR and via (c) RNA sequencing. Combined data from two independent experiments (n = 3 or 4 biological replicates) (b) and data from one experiment (n = 3 biological replicates) (c). Symbols represent biological replicates; bars represent the mean. Error bars represent mean ± SD.
