Extended Data Fig. 3: Targeting of STEEP by CRISPR.
From: STEEP mediates STING ER exit and activation of signaling
a, Illustration of gRNAs sequence and location used to target human STEEP. b, Immunoblot for STEEP in THP-1-derived clones transduced with STING-targeting gRNAs. c, STEEP KO THP-1 cells were transfected with mRNA encoding WT STEEP. 24 h later, the cells were lysed, and immunoblotted as shown. For comparison, lysates from WT THP-1 cells were included. d-f, Efficiency of STEEP gene targeting in primary human fibroblasts d, primary human macrophage e, and SAVI patient fibroblasts f. g, gRNAs used to target STEEP in mice. h-i, Efficiency of STEEP targeting in MEF cells. The KO scores were calculated based on the ICE Analysis tool from Synthego. In panels b and c, data shown are representative blots from three biologically independent experiments with similar results.
