Extended Data Fig. 4: Effect of STEEP KO on STING signaling.
From: STEEP mediates STING ER exit and activation of signaling
a, STEEP KO THP-1 cells electroporated with GFP mRNA, WT STEEP mRNA or mut5 STEEP mRNA were stimulated with cGAMP (150 nM, 1 h). Levels of pSTING and pTBK1 were monitored by Immunoblotting. (n = 3 biologically independent experiments). b-c, Immunoblot analysis of the indicated proteins from whole cell lysates of WT or STEEP KO Hela cells b, and human foreskin fibroblasts (c) after stimulation with dsDNA for the indicated time intervals. (n = 3 biologically independent experiments). All data shown in this figure are representative blots from three biologically independent experiments.
