Extended Data Fig. 5: The RK motif is important for ligand-induced recruitment of Lck to the TCR at 37 °C. | Nature Immunology

Extended Data Fig. 5: The RK motif is important for ligand-induced recruitment of Lck to the TCR at 37 °C.

From: Noncanonical binding of Lck to CD3ε promotes TCR signaling and CAR function

Extended Data Fig. 5

Proximity ligation assay between the TCR (CD3δ) and Lck was done as in Fig. 1. The indicated H8εsh cell variants were either left unstimulated (Uns), stimulated with 5 μg/ml of anti-mCD3ε antibody (145-2C11) or with 0.1 mM pervanadate (PerV) at 37 °C for 5 min. Data from five independent experiments were pooled and statistical analysis compared anti-mCD3ε stimulated H8εsh-CD3εWT cells and each of the H8εsh cell variants (grey bars) using unpaired Student’s t test. Mean values ± s.e.m. are shown; *P < 0.05; **P < 0.01; NS, not significant.

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