Extended Data Fig. 6: The CD3ε RK motif is required for thymic T cell development.
From: Noncanonical binding of Lck to CD3ε promotes TCR signaling and CAR function
a, Flow cytometric analysis of GFP expression levels in hematopoietic stem cells (HSC) from CD3ε-/- CD45.2+ donor mice transduced with lentiviruses encoding either mCD3εWT or mCD3εRKAA and IRES-GFP (black histograms). Non-transduced (NT) cells served as a control (grey histograms). b, Flow cytometric analysis of CD4 and CD8 surface expression on thymocytes isolated 6 weeks after injection of transduced CD3ε-/- CD45.2+ HSC into irradiated Rag2-/- recipient mice. GFP+ cells were gated and T cell development analyzed. As reference the thymus of a CD3ε-/- CD45.2+ mouse was analyzed (most right panel) c, The percent and d, the total number of thymocytes in the populations indicated was determined (DP: CD4+CD8+; DN: CD4−CD8−; CD4: CD4+CD8−; CD8: CD4−CD8+). e, Flow cytometric analysis of CD44 and CD25 surface expression on DN thymocytes. The DN populations were subdivided into DN1 to DN4 populations: DN1 (CD44+CD25−); DN2 (CD44+CD25+); DN3 (CD44−CD25+); and DN4 (CD44−CD25−). Representative dot plots are shown. Mean values ± s.e.m. of 9 mice are shown. Statistical analysis was performed using unpaired Student’s t-test. *P < 0.05; **P < 0.01; NS, not significant.
