Extended Data Fig. 5: NRP1 cell-intrinsically limits the in vivo persistence of antigen-specific CD8+ T cells.
From: Neuropilin-1 is a T cell memory checkpoint limiting long-term antitumor immunity
a, The level of Db-gp100+ transgenic TCR, activation status (CD44 vs. CD62L); and the ratio within donor pool (CD45.2+, recovered from spleen) of Nrp1–/– and Nrp1+/+ pMel-T cells 12 h after adoptive transfer, prior to B16-gp100 tumor inoculation (D0). b, Correlation between ratio of intratumoral Nrp1–/– vs. Nrp1+/+ donor pMel-T cells with tumor volume (mm3). c, Kinetics of tumor density (counts/mm3 tumor size) of intratumoral Nrp1–/– and Nrp1+/+ pMel-T cells during 1° B16-gp100 growth. (n = 5 for each time point) d, e Frequencies of Nrp1–/– and Nrp1+/+ pMel-T cells within the CD45.2+ donor pool in spleen and peripheral blood (PB) during (d) primary phase and (e) recall phase at the indicated time points (n = 5 for each time point). f, Representative flow cytometry plots for NRP1 expression on pMel-T cells of naïve, effector, MPECs, and TCM phenotypes, respectively. g, Frequencies of Nrp1+/+ or Nrp1–/– donor-derived cells of CD27+CD62L– (MPECs enriched) or CD27+CD62L+ (TCM) phenotype within CD45.2+ compartment over time, recovered from draining lymph nodes (DLN) and spleen from D12 to D42, n = 5 for each time point. h–i, Representative flow cytometry plots depicting the expression of Bcl2 against Ki67 with NRP1+ vs. NRP1– fractions (f) or Bcl2 vs. IRF4 in Nrp1+/+ or Nrp1–/– pMel-T cells (g) recovered from NdLN, on D12 and D21 post 1° B16-gp100 inoculation. Data in c–e and g were pooled from 2 independent time course cohorts. Error bars, mean±s.e.m; Statistical significance was determined by two-tailed paired Student’s t test (d, e) or two-way ANOVA (g), ****p < 0.0001.
