Extended Data Fig. 1: Characterization of NRP1 expression on CD8+ T cells in vivo.
From: Neuropilin-1 is a T cell memory checkpoint limiting long-term antitumor immunity
a, Gating strategy and phenotyping markers used for the identification of CD8+ cell subsets under conditions of homeostatic (naive mice), or sites of immunological challenge (LCMV infection or B16 tumor). b, Expression of NRP1 measured by flow cytometry in subsets of CD8ab+ T cells from spleens of mice infected by acute LCMV (Armstrong, D8 p.i, n = 7) or chronic LCMV (Clone 13, D30 p.i., n = 7). Bar graph tabulating the mean percentage of NRP1+ cells of indicated subsets and symbols represented individual mouse, 2 independent cohorts pooled. Error bars, mean±s.e.m; two-tailed unpaired Student’s t test. c, Representative flow cytometry plots depicting the co-expression of NRP1 and PD1 within naive (spleen-derived) and TEFF CD8+ cells (spleen or intratumoral) from unchallenged or B16-tumor bearing mice. d, Histograms depicting the correlation of NRP1 expressed by intratumoral CD8+ T cells with known markers of activation (CD44, CD69, CD25), cell proliferation (BrdU), naive/memory (CD62L, CD127) and senescence (KLRG1) phenotypes.
