Extended Data Fig. 2: Genetic models for the “loss- or gain-of function” manipulations of Nrp1 transcription restrictively in CD8+ T cells.
From: Neuropilin-1 is a T cell memory checkpoint limiting long-term antitumor immunity
a, b “Loss-of-function” model: Validation for the CD8-restricted Nrp1 deletion in the E8ICreNrp1L/L mice. (a) Representative histograms for NRP1 staining on CD4+ (green) and CD8+ (blue) tumor-infiltrating lymphocytes (TILs) of B16 tumors (D12). (b), Surface NRP1 expression on major immune cell populations derived from naive spleen (upper) or 1° B16 TILs (lower, D15 post implantation) of E8ICre or E8ICreNrp1L/L mice (n = 5 for each group). Loss of NRP1 protein restrictively by CD8+ cells in the E8ICreNrp1L/L mice was highlighted; c, “Gain-of-function” model: (Upper) Schematic structure of the Rosa26LSL.mAmetrine.2A.Nrp1 targeting construct. (Lower) Tamoxifen-induced CD8-specific (marked by GFP reporter) “constitutive” NPR1 expression (surface NRP1 staining) driven by Rosa26 promoter (marked by Ametrine reporter), in CD8+ T cells from peripheral blood (PB) of naïve mice or 1° B16 TILs. Gated on CD8+GFP+ cells.
