Extended Data Fig. 3: CD8-expressing NRP1 is dispensable for controlling the growth of primary tumors.
From: Neuropilin-1 is a T cell memory checkpoint limiting long-term antitumor immunity
a–e, B16 tumors implanted in the E8ICre or E8ICreNrp1L/L mice were harvested on D15, and the composition of major immune cell populations were analyzed by flow cytometry. (a) bh-SNE depiction of intratumoral CD45+ cells (80,233 in total, pooled from E8ICre and E8ICreNrp1L/L mice), projected by cell source. (b) Phenograph depiction of intratumoral CD45+ cells as in (a), and different cell lineages (colored) were identified by the expression of lineage-specific markers. (c) Percentage of major immune cell populations (indicated) within CD45+ TILs. (d) Numeration of CD45+ cells, presented by counts per gram tumor mass; Error bars, mean±s.e.m; Symbol represented individual mouse (n = 5 for each genotype). (e–h) Growth curves for (e) 1° B16 tumor; (f) B16-OVA tumor (Vaccination with attenuated Listeria monocytogenes expressing Ova peptide (LM-Ova) was given on Day 4) (g) 2° B16 tumor in the E8ICre and E8ICreNrp1L/L mice, challenged on Day 30 (left) or 60 (right); (h) 1° and 2° B16 tumors (+30d rechallenge) implanted in the E8ICreErt2gfpRosa26LSL.Nrp1 and E8ICreErt2gfp mice. Data were representative of 2 independent experiments (e, f); or pooled from 4 independent experiments (g, h). Error bars, mean±s.e.m; Statistical significance was determined by two-tailed unpaired Student’s t test (d) or two-way ANOVA with correction for multiple comparisons (e–h).
