Extended Data Fig. 5: Neutrophil EGF regulates DC-differentiation of monocytes.
From: In situ neutrophil efferocytosis shapes T cell immunity to influenza infection
a, Immunofluorescence image of a permeabilized neutrophil stained with Abs against Ly6G (green) and EGF (red). Scale bar, 5 μm. b, Flow cytometry analysis of EGF in permeabilized mouse neutrophils (day 4 post-infection). c, Secretion of EGF from neutrophils undergoing spontaneous apoptosis. d, Flow cytometry analysis of phagocytic activity (tdTomato) of monocytes (Csf1r-GFP) isolated from the influenza infected lung after co-incubation with tdTomato+ apoptotic neutrophils for 12 h in the presence of PBS or erlotinib (0.1 μg/ml). e, Monocytes (Csf1r-GFP) were isolated from the influenza infected lung. After co-incubation with tdTomato+ apoptotic neutrophils for 12 h in the presence of PBS or erlotinib (0.1 μg/ml), neutrophil-engulfed (tdTomato+) and non-engulfed (tdTomato−) monocytes were sorted, pulsed with OVA peptide, and incubated with CD8+ T cells isolated from influenza-infected OT-I mouse dLNs. Monocyte-T cell conjugation was measured by microscopy. Data are presented as the mean ± SEM, n ≥ 3 mice per group. Statistical differences were assessed by nonparametric Mann-Whitney test.
