Extended Data Fig. 2: Basophil depletion results in elevated ILC2 responses.
From: Basophils prime group 2 innate lymphoid cells for neuropeptide-mediated inhibition
a, Lung neutrophils and (b), eosinophils were quantified by flow cytometry on day 7 post-Nb infection in control or baso-dep mice. c, Representative flow cytometric gating strategy to evaluate neutrophils and eosinophils. d, ILC2s in the lung were quantified on day 7 post-Nb infection in control or baso-dep mice. Intracellular cytokine staining for (e, f), IL-5 and IL-13 was performed on lineage negative, CD90+, CD127+ ILC2s in lung on day 7 post-Nb infection and cytokine positive cells were quantified. g, Representative flow cytometric gating strategy to evaluate ILC2 populations. P values were determined by two-tailed Student’s t-tests. *P < 0.05, **P < 0.01, ***P < 0.001. a-g, Representative of at least 3 separate experiments with at least 5 mice per group.
