Extended Data Fig. 7: NUR77/Nr4a1 and NOR-1/Nr4a3 redundantly restrain BCR-induced B cell expansion.

a-c, Samples correspond to those described in Fig. 5e,f. a, Histograms depict CTV dilution in CD45.2 + B cells (black histogram) and co-cultured CD45.1 WT B cells (shaded gray histogram), and are representative of at least 3 mice/genotype. b, Shown is the ratio of CD45.2+ WT, Nr4a1^−/− or Nr4a3^−/− B cells relative to co-cultured CD45.1+ WT B cells (+ 20 ng/ml BAFF), normalized to the unstimulated condition. c, Graph depicts division index for each genotype. d, e, Samples correspond to those described in Fig. 5i,j, cultured in the presence of 20 ng/ml BAFF. d, Shown is the ratio of each CD45.2 + B cell genotype relative to co-cultured CD45.1+ WT B cells, normalized to the unstimulated condition. e, Graph depicts division index for each genotype. f-k, Competitive bone marrow chimeras were generated as described in Extended Data Fig. 5h, i. 10-12 weeks after reconstitution, lymphocytes were harvested from chimeras, CTV loaded and cultured with given stimuli (anti-IgM doses + /- 20 ng/ml BAFF + /- 10 ng/ml IL-4). N = 5 chimeras of each genotype were analyzed. f, g, h, Graphs depict ratio of CD45.2+ cre+ or cDKO B cells relative to CD45.1/2 WT B cells from each chimera, normalized to unstimulated condition. i, j, k, Graphs depict division index for CD45.2+ cre+ or cDKO B cells from each chimera. Graphs in this figure depict N = 3 biological replicates for panels (D-E) except (F-K) as noted above which represent N = 5 chimeras each. Mean + /- SEM displayed for all graphs. Statistical significance was assessed with two-way ANOVA with Tukey’s (B-E); two-tailed unpaired student’s t-test with Holm-Sidak (F-K). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.